Crystal Violet Cell Cytotoxicity Assay Kit (BN00596)

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SKU:
BN00596
  • Crystal Violet Cell Cytotoxicity Assay Kit (BN00596)
  • Crystal Violet Cell Cytotoxicity Assay Kit (BN00596)
€420

Description

ELISA Kit Technical ManualMSDS

Crystal Violet Cell Cytotoxicity Assay Kit

Crystal violet (CV) cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. CV is a triarylmethane dye that can bind to ribose type molecules such as DNA in nuclei. Normally, dead adherent cells will detach from cell culture plates and will be removed from viable cell propulation during washing steps. CV staining can be used to quantify the total DNA of the remaining population and thus determine cell viability. The CV staining is directly proportional to the cell biomass and can be measured at 570 nm. CV staining is a quick and versatile assay for screening cell viability under diverse stimulation or inhibition conditions. Assay Genie's CV cell cytotoxicity assay kit is simple, accurate, reproducible and sensitive. It also includes Doxorubicin as a positive control. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening

Figure: (a) Dose-response curve of HepG2 (b) and MCF-7 (b) cells to doxorubicin for 72 hr determined by the CV staining assay. Assays were performed according to the kit protocol in triplicate.

Key Information Description

Product SKU

BN00596

Size

1000 assays

Kit Summary

  • Detection method- Absorbance (570 nm)
  • Sample type- Cell culture (adherent and non adherent)
  • Species reactivity- Mammalian
  • Applications- This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of crystal violet to bind ribose molecules (i.e. DNA) in viable cells.

Detection Method

Detection method- Absorbance (570 nm)

Species Reactivity

Mammalian

Applications

This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays.This assay relies on the ability of crystal violet to bind ribose molecules (i.e. DNA) in viable cells.

Features and Benefits

  • Simple procedure; takes less than 2 hours
  • Fast, sensitive and convenient

Kit Components

Crystal Violet Staining Solution

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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