Dihydrofolate Reductase Activity Kit (Colorimetric) (BN00511)

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BN00511
  • Dihydrofolate Reductase Activity Kit (Colorimetric) (BN00511)
  • Dihydrofolate Reductase Activity Kit (Colorimetric) (BN00511)
  • Dihydrofolate Reductase Activity Kit (Colorimetric) (BN00511)
  • Dihydrofolate Reductase Activity Kit (Colorimetric) (BN00511)
$621.18

Description

ELISA Kit Technical ManualMSDS

Dihydrofolate Reductase Activity Kit (Colorimetric)

Dihydrofolate Reductase (DHFR; 5,6,7,8-tetrahydrofolate NADP oxidoreductase; EC 1.5.1.3), is a ubiquitous enzyme that is present in all eukaryotic and prokaryotic cells. It catalyzes the reduction of dihydrofolate (FH2) to tetrahydrofolate (FH4) using NADPH as a cofactor. FH4 is essential for a number of enzymes that are necessary for the de novo synthesis of purines, thymidylic acid and some amino acids. Inactivation of the DHFR enzymatic activity causes reduction of the intracellular level of FH4, inhibition of RNA and DNA synthesis, and cell death. For this reason, DHFR has been a critically important enzyme as a molecular target in drug discovery. Assay Genie's Dihydrofolate Reductase assay kit is based on the ability of DHFR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. Our assay has been optimized to be carried out in a 96- well plate. The assay is simple, sensitive and can detect as low as 4 mU/ml in a variety of samples.

Figure: (a) NADPH standard curve; (b) Purified Dihydrofolate Reductase activity; (c) Dihydrofolate Reductase activity in Rat Liver (100 µg); (d) Dihydrofolate Reductase specific activity was calculated from Rat Liver (100 µg) or Hela cells (80 µg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00511

Size

100 assays

Detection Method

Absorbance (OD=340 nm)

Species Reactivity

Mammalian

Applications

Detection of Dihydrofolate Reductase activity

Features and Benefits

  • Rapid, sensitive and convenient
  • The kit measures total Activity of DHFR Activity with limit of quantification 4 mU/ml using recombinant DHFR

Kit Components

  • DHFR Assay Buffer
  • DHFR Substrate
  • Dihydrofolate Reductase
  • NADPH

Storage Conditions

-70°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans.

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