Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit (BN00897)

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BN00897
  • Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit (BN00897)
  • Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit (BN00897)
€876

Description

ELISA Kit Technical ManualMSDS

Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit

Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes. Assay Genie's Dihydroxyacetone Phosphate Assay kit is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, TPI converts DHAP to GAP that undergoes a series of reaction and reduces to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of Dihydroxyacetone Phosphate. This simple, sensitive, and easy to use assay kit can detect Dihydroxyacetone Phosphate as low as 0.5 µM in a variety of samples.

Figure: (a) Dihydroxyacetone Phosphate Standard Curve, (b) Measurement of Dihydroxyacetone Phosphate level in Jurkat (250 µg), and MCF-7 (150 µg) cell lysate and in rat liver (50 µg) and muscle (150 µg) lysate.

Key Information Description

Product SKU

BN00897

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

  • Measurement of Dhydroxyacetone Phosphate in various tissues/cells.
  • Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis.
  • Mechanistic study of human TPI deficiency

Features and Benefits

  • Simple, Sensitive & Rapid protocol.
  • Highly-Sensitive: Detects as low as 0.5 µM DHAP

Kit Components

  • DHAP Assay Buffer (in DMSO)
  • DHAP Enzyme Mix
  • DHAP Developer
  • DHAP Standard

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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