Factor Xa Inhibitor Screening Kit (Fluorometric) (BN00625)

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SKU:
BN00625
  • Factor Xa Inhibitor Screening Kit (Fluorometric) (BN00625)
  • Factor Xa Inhibitor Screening Kit (Fluorometric) (BN00625)
€726

Description

ELISA Kit Technical ManualMSDS

Factor Xa Inhibitor Screening Kit (Fluorometric)

Factor Xa (FXa) is the activated form of the coagulation factor X (Stuart-Power factor, thrombokinase, prothrombinase, thromboplastin, E.C.3.4.21.6). Factor X, a serine endopeptidase, plays an important role at several stages of the coagulation pathway. It acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor Antithrombin to inactivate several coagulation factors including factor Xa. Assay Genie's Factor Xa inhibitor screening kit utilizes the ability of Factor Xa to cleave a synthetic substrate thereby releasing a fluorophore, AMC, which can be quantified by a fluorescence reader. In the presence of an inhibitor, the extent of the cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The Factor Xa Inhibitor Screening Kit is a simple, straightforward, high-throughput assay to screen Factor Xa inhibitors.

Figure: (a) Inhibition of FXa activity by FXa Inhibitor (GGACK Dihydrochloride). (b) FXa activity was measured in plasma samples in the presence and absence of FXa Inhibitor (GGACK Dihydrochloride). S = Substrate, I = Inhibitor. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00625

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 350/450 nm)

Applications

  • Screening potential inhibitors of Factor Xa
  • Characterizing/studying Factor Xa inhibitors in plasma samples

Features and Benefits

  • Simple and reliable test to screen/study/characterize Fxa inhibitors
  • High-throughput
  • Includes Inhibitor Control (GGACK Dihydrochloride)

Kit Components

  • FXa Dilution Buffer
  • FXa Assay Buffer
  • FXa Enzyme
  • FXa Substrate
  • FXa Inhibitor (GGACK Dihydrochloride, 10 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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