Factor XIa Activity Assay Kit (Colorimetric) (BN01132)

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BN01132
  • Factor XIa Activity Assay Kit (Colorimetric) (BN01132)
  • Factor XIa Activity Assay Kit (Colorimetric) (BN01132)
€702

Description

ELISA Kit Technical ManualMSDS

Factor XIa Activity Assay Kit (Colorimetric)

Factor XI or plasma thromboplastin antecedent (EC 3.4.21.27), the zymogen form of factor XIa, is a serine protease. In humans, Factor XI is encoded by the F11 gene. Factor XI (FXI) circulates as a homo-dimer in its inactive form activated into factor XIa by factor XIIa (FXIIa) via the "contact pathway", thrombin, and self-activation by its active form (FXIa). The deficiency of factor XI causes the rare hemophilia C which is an autosomal recessive disorder characterized by lower spontaneous bleeding, but excessive blood loss during surgical procedures. Low levels of factor XI also occur in many other disease states, including Noonan syndrome. High levels of factor XI have been implicated in thrombosis. Factor XIa also activates factor IX which, in turn, activates factor X in the coagulation cascade. Assay Genie's Factor XIa activity assay kit utilizes the ability of factor XIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD 405 nm). The kit is easy-to-use and can detect Factor XIa (as low as 1 mPEU) from plasma and purified protein samples.

Figure: Kinetic progressive curves for different amounts of FXIa Enzyme (A) and Activated Plasma Samples (B) are shown. Standard curve for pNA (n = 3) (C) was used to estimate FXIa activity in plasma (n = 5) (D). Assays were performed according to the kit protocol.

Key Info Description

Product SKU

BN01132

Size

100 assays

Detection Method

Colorimetric (Absorbance 405 nm)

Species Reactivity

Mammalians

Applications

Measurement of Factor XIIIa activity in plasma samples and purified/crude enzyme preparations

Features & Benefits

  • Simple, rapid & convenient assay to measure Factor XIa
  • Includes Positive Control and Factor Xia and Factor XIIa Inhibitors

Kit Components

  • FXIa Assay Buffer
  • FXIa Activator
  • Inhibitor 1
  • Inhibitor 2
  • FXIa Substrate
  • Human Factor XIa
  • pNA Standard (0.1 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans

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