Factor XIIa Activity Assay Kit (Colorimetric) (BN01152)

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BN01152
  • Factor XIIa Activity Assay Kit (Colorimetric) (BN01152)
  • Factor XIIa Activity Assay Kit (Colorimetric) (BN01152)
$794.17

Description

ELISA Kit Technical ManualMSDS

Factor XIIa Activity Assay Kit (Colorimetric)

Factor XII or Hageman factor (EC 3.4.21.38), is the zymogen form of factor XIIa, a serine protease involved in Coagulation Pathway.This single chain zymogen is activated by Kallikrein into a two-chain serine protease (XIIa) with a heavy chain (factor XIIa-alpha) and a light chain. Human Factor XII and Prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates Prekallikrein to Kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. The defects in this gene do not cause any clinical symptoms but prolong the whole blood clotting time. Assay Genie's Factor XIIa activity assay kit utilizes the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma samples.

Figure: (A) Kinetic progressive curves for different amounts of FXIIa Enzyme and (B)Activated Plasma Samples are shown. (C) Standard curve for pNA (n = 3) was used to (D) estimate FXIIa activity in Normal Pooled Human Plasma and FXII-deficient Human Plasma (n = 3) . Assays were performed according to the kit protocol.

Key Info Description

Product SKU

BN01152

Size

100 assays

Detection Method

Colorimetric (Absorbance 405 nm)

Species Reactivity

Mammalians

Applications

Measurement of Factor XIIa activity in plasma samples and purified/crude enzyme preparations

Features & Benefits

  • Simple, rapid & convenient assay to measure Factor XIa
  • Includes Positive Control and Factor XIIa and Factor XIIa Inhibitors

Kit Components

  • FXIIa Assay Buffer
  • FXIIa Activator
  • FXIIa Substrate
  • Human Factor XIIa
  • Human Factor XIIa Supplement
  • FXIIa Inhibitor
  • pNA Standard (0.1 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans.

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