Formaldehyde Fluorometric Assay Kit (BN01021)

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SKU:
BN01021
  • Formaldehyde Fluorometric Assay Kit (BN01021)
  • Formaldehyde Fluorometric Assay Kit (BN01021)
€475

Description

ELISA Kit Technical ManualMSDS

Formaldehyde Fluorometric Assay Kit

Formaldehyde (HCHO) is the simplest aldehyde and one of the most often used organic compounds in industrial processes due to its high reactivity with a variety of chemicals. Endogenous HCHO can be produced in organisms via metabolic processes while exogenous HCHO can be absorbed after oral, dermal or inhalation exposure. HCHO is highly toxic due to its capacity to covalently bind to macromolecules, such as DNA, and is well known for its neurotoxicological effects. Exposure to relatively high levels of HCHO is believed to cause leukemia, nose and nasopharyngeal cancer, etc.; however the epidemiological evidence is unclear. It can also affect memory and learning capacity. Assay Genie's Formaldehyde Assay kit provides a simple, sensitive, and high-throughput adaptable assay that detects biologically relevant concentrations of HCHO in various fluids and tissues. The assay is based on the oxidation of HCHO, producing a stable fluorescent signal, which is directly proportional to the amount of HCHO in samples. The kit can detect less than 2 µM of HCHO in a variety of samples.

(a)

(b)

Figure: (a) HCHO Standard Curve. (b) Measurement of Formaldehyde concentration in human urine from non-diabetic and diabetic donors. Samples were eproteinized using 10 kDa Spin Column and diluted 10X. Diluted samples (20 μl) were spiked with known amount of HCHO (300 pmol). Sample preparation and assay was performed following kit protocol.

Key Information Description

Product SKU

BN01021

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

Measurement of Formaldehyde in various biological samples

Features and Benefits

  • Rapid, simple & sensitive
  • High-throughput adaptable
  • The kit can detect less than 2 µM of HCHO in a variety of samples.

Kit Components

  • HCHO Assay Buffer (in DMSO)
  • HCHO Enzyme Mix (Lyophilized)
  • HCHO Developer (Lyophilized)
  • HCHO Standard (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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