Free Fatty Acid Colorimetric Assay Kit (384-well) (BN01116)

  • Metabolism Assays Free Fatty Acid Colorimetric Assay Kit 384-well BN01116
  • Metabolism Assays Free Fatty Acid Colorimetric Assay Kit 384-well BN01116


ELISA Kit Technical ManualMSDS

Free Fatty Acid Colorimetric Assay Kit (384-well)

Fatty Acids play important roles in normal metabolism and in many disease states. They are precursors to a number of bioactive classes of compounds including prostaglandins and leukotrienes, and have been implicated to play roles in such diverse functions as the immune system, autism, and the inflammatory response. Fatty acid levels are an important measure of the physiologic state. High levels are associated with diabetes mellitus, obesity, endocrine dysfunctions, and metabolic syndrome. Assay Genie's Free Fatty Acid Colorimetric assay kit provides a simple method for the quantification free fatty acids. The kit offers a convenient, sensitive, enzyme-based method for the detection of long-chain (C-8 (octanoate) and longer) fatty acids in various biological samples. In the assay, fatty acids are converted to their CoA derivatives, which are subsequently oxidized by the enzyme mix to yield an intermediate. Formed intermediate then reacts with the Free Fatty acid probe to generate color which can be read by a spectrophotometer at OD 570 nm. Our 384-well format enables analyses of large number of samples on a single plate in a high throughput mode. Sample volumes between 1 and 10 can be used with this kit

Figure: (a) Palmitic Acid Standard Curve. (b) Determination of Free Fatty Acid in pooled normal human serum. Undiluted serum sample (2 µl, untreated) was assayed directly according to the kit protocol. Calculated Free Fatty Acid concentration: 312 ± 4.2 μM.

Key Information Description

Product SKU



400 assays

Detection Method

Absorbance (570 nm)

Species Reactivity



The kit has can detect physiological concentration of Free Fatty Acids in samples

Features and Benefits

  • Simple procedure; takes ~40 minutes
  • Fast and convenient
  • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples

Kit Components

  • Fatty Acid Assay Buffer
  • Fatty Acid Probe (in DMSO, anhydrous)
  • ACS Reagent (lyophilized)
  • Enzyme Mix (lyophilized)
  • Enhancer
  • Palmitic Acid Standard (1nmol/µl)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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