Glucokinase Activity Assay Kit (Fluorometric) (BN01129)

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BN01129
  • Glucokinase Activity Assay Kit (Fluorometric) (BN01129)
  • Glucokinase Activity Assay Kit (Fluorometric) (BN01129)
  • Glucokinase Activity Assay Kit (Fluorometric) (BN01129)
€679

Description

ELISA Kit Technical ManualMSDS

Glucokinase Activity Assay Kit (Fluorometric)

Glucokinase (also called GCK, hexokinase type IV or D and ATP: D-hexose 6-phosphotransferase; EC 2.7.1.1) is expressed in specific types of tissues: liver, pancreas, small intestine and brain. Glucokinase functions as a glucose sensor, triggering shifts in carbohydrate metabolism or cell function in response to the levels of glucose in blood, such as nutritional and hormonal molecular pathways. Unlike other Hexokinases, Glucokinase has a relatively low affinity for glucose and it is not inhibited by physiological concentrations of glucose 6-phosphate. Mutations in the gene encoding GCK can cause both hyperglycemia and hypoglycemia. Due to the major role of Glucokinase in controlling blood glucose homeostasis, Glucokinase is currently considered as a strong candidate target for the treatment of Hyperglycemia, a condition encountered in Type 2 Diabetic patients. Assay Genie's Glucokinase Activity Assay Kit provides a quick and easy method for monitoring GCK activity in wide variety of samples. In this assay, GCK converts glucose into glucose-6-phosphate, which in turn is converted into a series of intermediates that reduce generating an intense fluorescence product (Ex/Em=535/587nm). The assay is simple, specific, sensitive and high-throughput adaptable and can detect as low as 2 µU of GCK activity.

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(b)

(c)

Figure: (a) NADPH Standard Curve. (b) GCK Activity in Mouse Liver. (c) Measurement of GCK activity in Mouse Liver tissue extracts (2 µg protein); Rat Liver tissue extracts (5 µg protein) and Hep G2 Cell Lysates (2 µg protein). All assays were performed following kit protocols.

Key Info Description

Product SKU

BN01129

Size

100 assays

Kit Summary

  • Detection method- Fluorescence (Ex/Em = 535/587 nm)
  • Applications-Measurement of Glucokinase activity in various tissues/cells.
  • Analysis of Glucose metabolism and Cell signaling in various cell types.
  • Screening anti-diabetic drugs.

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Species Reactivity

Eukaryotes

Applications

  • Measurement of Glucokinase activity in various tissues/cells.
  • Analysis of Glucose metabolism and Cell signaling in various cell types.
  • Screening anti-diabetic drugs.

Features & Benefits

  • Simple and rapid procedure
  • Highly Sensitive
  • Can detect glucokinase activity even as low as 2 µU

Kit Components

  • GCK Assay Buffer (in DMSO)
  • DTT (1 M)
  • GCK Substrate
  • Sample Background Reagent
  • ATP
  • GCK Enzyme Mix
  • GCK Developer
  • GCK Positive Control
  • NAPDH Standard (200 nmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans

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