Glucose Colorimetric Assay Kit (384 Well) (BN01111)

SKU:
BN01111
Product type:
Cellular Assay
  • Metabolism Assays Glucose Colorimetric Assay Kit 384 Well BN01111
  • Metabolism Assays Glucose Colorimetric Assay Kit 384 Well BN01111
€682

Description

ELISA Kit Technical ManualMSDS

Glucose Colorimetric Assay Kit (384 Well)

Glucose (C6H12O6, FW: 180.16) is delivered to the cells from the blood stream as the primary biological fuel to generate the universal energy molecule, ATP. Even though human body has evolved an advanced metabolic pathway to keep the blood glucose level in normal range, failure happens and that leads to develop conditions of persistent high or low blood sugar. Diabetes mellitus is one such most important metabolic disorder where an increased blood-sugar level develops compare to normal blood sugar level. Glucose level in blood is also a key diagnostic parameter for many metabolic disorders and its measurement is very important in both research and drug discovery. Assay Genie's Glucose Colorimetric Assay Kit, uses a glucose enzyme mix which oxidizes glucose specifically and produces a product which reacts with a chromophore generating a stable signal (OD: 590 nm). This color is directly proportional to the amount of glucose present in the sample. The method is quantitative, rapid, simple, sensitive, and designed to be used in high throughput settings. The kit can detect 0.5 to 5 mM of glucose in various biological samples

Figure: (a) Glucose Standard Curve; (b) Quantitation of Glucose in human serum. Serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Undiluted serum filtrate (1 µl) samples were added to the wells directly. Assays were performed according to the kit protocol. Calculated concentration: 86.78 ± 0.1 mg/dl.

Key Information Description

Product SKU

BN01111

Size

384 assays

Detection Method

Absorbance (590 nm)

Species Reactivity

Mammalian

Applications

The kit detects 0.5-500 µM glucose samples.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Glucose Assay Buffer
  • Glucose Probe Glucose
  • Enzyme Mix (lyophilized)
  • Glucose Standard (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.