Glucose Colorimetric/Fluorometric Assay Kit (BN00830)

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SKU:
BN00830
  • Glucose Colorimetric/Fluorometric Assay Kit (BN00830)
  • Glucose Colorimetric/Fluorometric Assay Kit (BN00830)
  • Glucose Colorimetric/Fluorometric Assay Kit (BN00830)
€589

Description

ELISA Kit Technical ManualMSDS

Glucose Colorimetric/Fluorometric Assay Kit

Glucose (CO FW: 180.16) is a very important fuel source to generate universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes. The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.

Figure: Glucose Standard Curve; (a) Colorimetric (b) Fluorometric, (c) Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Assays were performed according to the kit protocol. Calculated concentrations: Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.

Key Information Description

Product SKU

BN00830

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Application

The kit detects 1-10000 µM glucose samples.

Features and Benefits

  • Simple procedure; takes ~40 minutes
  • Fast and convenient
  • Kit provides all necessary buffers and reagents for a accurate assay of glucose in various mammalian samples.

Kit Components

  • Glucose Assay Buffer
  • Glucose Probe (in DMSO)
  • Glucose Enzyme Mix (lyophilized)
  • Glucose Standard (100 nmol/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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