Glucose Uptake Colorimetric Assay Kit (BN00900)

SKU:
BN00900
  • Metabolism Assays Glucose Uptake Colorimetric Assay Kit BN00900
  • Metabolism Assays Glucose Uptake Colorimetric Assay Kit BN00900
€1,197

Description

ELISA Kit Technical ManualMSDS

Glucose Uptake Colorimetric Assay Kit

Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In Assay Genie's glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.

Figure 1. (a) 2-DG6P Standard curve (b) 2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. Assays were performed following kit protocol. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.

Key Information Description

Product SKU

BN00900

Size

100 assays

Detection Method

Absorbance (412 nm)

Species Reactivity

Mammalian

Applications

This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • The assay is easy, non-radioactive, and highly sensitive

Kit Components

  • Extraction Buffer
  • Neutralization Buffer
  • 2-Deoxyglucose (2-DG, 10 mM)
  • Assay Buffer
  • Enzyme Mix (lyophilized)
  • Recycling Mix (lyophilized)
  • 2-DG6P Standard (lyophilized)
  • Glutathione Reductase (lyophilized)
  • Substrate-DTNB (lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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