Glutathione Peroxidase Activity Colorimetric Assay Kit (BN00975)

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SKU:
BN00975
  • Glutathione Peroxidase Activity Colorimetric Assay Kit (BN00975)
  • Glutathione Peroxidase Activity Colorimetric Assay Kit (BN00975)
  • Glutathione Peroxidase Activity Colorimetric Assay Kit (BN00975)
€589

Description

ELISA Kit Technical ManualMSDS

Glutathione Peroxidase Activity Colorimetric Assay Kit

Glutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. Assay Genie's Glutathione Peroxidase Assay Kit measures glutathione peroxidase (GPx) activity through a coupled reaction with glutathione reductase (GR). In the assay, GPx reduce Cumene Hydroperoxide, and oxidize GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH is proportionally to GPx activity in the reactions. The decrease of NADPH can be easily measured by absorbance at 340 nm. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with detection sensitivity ~ 0.5 mU/ml of GPx in samples.

Figure: (a) NADPH Standard Curve, (b) Measurement of GPx activity using purified enzyme, (c) GPx Activity was measured using rat liver lysate (23 µg), human serum (1 µl) and HeLa cell lysate (16 µg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00975

Size

100 assays

Detection Method

Absorbance (340 nm)

Species Reactivity

Mammalian

Applications

The assay has a detection sensitivity ~ 0.5 mU/ml of GPx in samples.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • GPx Assay Buffer
  • NADPH (lyophilized)
  • Glutathione Reductase
  • Glutathione (GSH, lyophilized)
  • Cumene Hydroperoxide
  • GPx Positive Control (lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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