Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric) (BN00678)

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BN00678
  • Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric) (BN00678)
  • Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric) (BN00678)
  • Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric) (BN00678)
  • Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric) (BN00678)
€649

Description

ELISA Kit Technical ManualMSDS

Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric)

Glycerophosphorylcholine (GPC), also referred to as alpha-GPC or choline alfoscerate, is a small metabolite and potential dementia treatment. GPC is a breakdown product of phosphatidylcholine, and dysregulation of phospholipid metabolism can alter lipid and metabolite concentrations, leading to health problems such as fatty liver, Gaucher's disease, and Tay-Sachs disease. GPC is also component of seminal fluid and breast milk, and a precursor of the neurotransmitter, acetylcholine. As GPC can efficiently cross the blood-brain barrier, it has been hypothesized as a treatment for Alzheimer's disease and other forms of dementia. In such cases, GPC serves as a biomarker for lipid membrane decomposition. Assay Genie's Glycerophosphorylcholine Assay Kit allows a quick and sensitive means to determine GPC concentration in a biological sample. The assay is robust and specific, with a GPC enzyme mix converting GPC in the sample into an intermediate that is then utilized to quantitatively generate signal using the GPC Developer mix and GPC Probe. The included Sample Clean-Up Mix allows the user to avoid high background from choline, which is present at high levels in many biological fluids. The assay is rapid, simple, high throughput compatible, and can detect GPC concentrations as low as 0.1 µM in tissue lysates and other samples such as breast milk and seminal plasma.

Figure: (a) GPC standard curve, colorimetric and (b) fluorometric. (c) Determination of total GPC concentration in Liver lysate, Human Milk, and Seminal Fluid. For this experiment, 100 mg rat liver was homogenized and prepared according to the kit protocol. Breast Milk and Seminal Plasma were prepped according to the above protocol and treated with Sample Clean-Up Mix. Values were determined with fluorometric measurements and are mean ± standard deviation of at least three independent determinations. (d) Specificity of the assay; PC = phosphatidylcholine, PA = phosphatidic acid, SM = sphingomyelin, GPC = glycerophosphorylcholine.

Key Information Description

Product SKU

BN00678

Size

100 assays

Kit Summary

  • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em= 535/587 nm)
  • Species reactivity- Eukaryotes
  • Applications- Assay Genie's Glcyerophosphorylcholine kit measures GPC levels as low as 0.1 µM

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em= 535/587 nm)

Species Reactivity

Eukaryotes

Applications

  • Measurement of GPC content of various tissues/cell extracts
  • Determination of GPC concentration in biological fluids

Features and Benefits

  • Simple procedure; takes less than ~40 minutes
  • Fast and convenient

Kit Components

  • GPC Assay Buffer
  • GPC Enzyme Mix
  • GPC Developer
  • GPC Probe (in DMSO)
  • Sample Clean-Up Mix
  • GPC Standard (1 µmole)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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