Glycogen Colorimetric Assay Kit (384-Well) (BN01120)

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SKU:
BN01120
  • Glycogen Colorimetric Assay Kit (384-Well) (BN01120)
  • Glycogen Colorimetric Assay Kit (384-Well) (BN01120)
€738

Description

ELISA Kit Technical ManualMSDS

Glycogen Colorimetric Assay Kit (384-Well)

Glycogen is a branched polysaccharide, made of glucose units that serves as the primary source of energy storage in animals. Most of the glucose residues in glycogen are linked by 1,4-glycosidic bonds with branching via 1,6 linkage. In mammals, the two major sites of glycogen storage are liver and skeletal muscle. In liver, glycogen synthesis and degradation are regulated to maintain blood-glucose levels, while stored glycogen in muscle can be processed to meet the energy needs of the muscle itself. Abnormal ability to utilize glycogen is found in patients suffering diabetes and other genetic glycogen storage related diseases. Assay Genie Glycogen Colorimetric Assay Kit is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen releasing glucose which is then specifically oxidized to produce a product that reacts with GenieRed Probe to generate color (OD 590 nm). The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The assay can detect as low as 0.025 µg of glycogen in 384-well assay format.

Figure: (a) Glycogen Standard Curve: (b) Glycogen in rat liver lysate. Rat liver (17 mg) was homogenized with 680 µl of deionized water, boiled for 10 min, and then centrifuged for 10 min. at 18000 x g. Supernatant was collected. Supernatant was diluted 10 times and 5 µl was used for the assay following the kit’s protocol. Sample was spiked with 0.2 µg of Glycogen Standard.

Key Information Description

Product SKU

BN01120

Size

400 assays

Detection Method

Absorbance (590 nm)

Species Reactivity

The assay can detect glycogen as low as 0.025 µg

Applications

  • Measurement of Glycogen in various tissues/cells
  • Analysis of metabolism an cell sginaling in various cells

Features and Benefits

  • Simple procedure; takes ~ 2 hours
  • Fast and convenient
  • Kit contains all necessary reagents for accurate measurement of Glycogen

Kit Components

  • Hydrolysis Buffer
  • Development Buffer
  • GenieRed Probe
  • Hydrolysis Enzyme Mix
  • Development Enzyme Mix
  • Glycogen Standard (2.0 mg/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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