HAT (H4) Activity Fluorometric Assay Kit (BN00602)

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BN00602
  • HAT (H4) Activity Fluorometric Assay Kit (BN00602)
  • HAT (H4) Activity Fluorometric Assay Kit (BN00602)
€651

Description

ELISA Kit Technical ManualMSDS

HAT (H4) Activity Fluorometric Assay Kit

Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. Assay Genie's HAT Activity Assay Kit utilizes Acetyl CoA and H4 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer and to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity equivalent to as low as 1.5 µU of p300 in a variety of samples.

Figure 1: (a) CoASH Standard Curve. (b) Measurement of HAT Activity: p300 (72 ng) or HeLa nuclear extract (positive control) as per kit protocol.

Key Information Description

Product SKU

BN00602

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

  • Measurement of HAT activity of immuno precipitated samples
  • Measurement of HAT activity of purified enzyme preparations
  • Measurement of HAT activity in Nuclear Extracts.

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect HAT Activity equivalent to as low as 1.5 µU of p300 in a variety of samples.

Kit Components

  • HAT Assay Buffer
  • Acetyl CoA (Lyophilized)
  • H4 Peptide (Lyophilized)
  • Substrate Mix (Lyophilized)
  • Developer
  • CoA Standard (Lyophilized)
  • Positive Control (HeLa Nuclear Extract)

Storage Conditions

-80°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans.

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