HDAC1 Immunoprecipitation (IP) & Activity Assay Kit (BN00606)

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SKU:
BN00606
  • HDAC1 Immunoprecipitation (IP) & Activity Assay Kit (BN00606)
  • HDAC1 Immunoprecipitation (IP) & Activity Assay Kit (BN00606)
€931

Description

ELISA Kit Technical ManualMSDS

HDAC1 Immunoprecipitation (IP) & Activity Assay Kit

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.

Figure: (a). AFC standard curve (b).HDAC1 IP Activity Assay of HeLa cell lysate and Nuclear Extract as per the kit protocol & Total HDAC Activity of Positive Control

Key Information Description

Product SKU

BN00606

Size

25 assays

Detection Method

Fluorescence (Ex/Em=380/500 nm)

Applications

  • Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s)
  • Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme
  • Screening for activators or inhibitors of HDAC1

Features and Benefits

  • Simple, fast and convenient.
  • Only kit on the market for both IP as well as measuring activity of HDAC1

Kit Components

  • HDAC Assay Buffer
  • Cell Lysis Buffer
  • HDAC Substrate
  • Developer
  • AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM)
  • Rabbit HDAC2 Antibody (0.2 mg/ml)
  • Rabbit IgG (Control Antibody) (0.5 mg/ml)
  • Protein A/G Sepharose Beads
  • Positive Control (Jurkat Cell Lysate) (125 )

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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