HDAC3 Immunoprecipitation (IP) & Activity Assay Kit (BN00608)

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SKU:
BN00608
  • HDAC3 Immunoprecipitation (IP) & Activity Assay Kit (BN00608)
  • HDAC3 Immunoprecipitation (IP) & Activity Assay Kit (BN00608)
€852

Description

ELISA Kit Technical ManualMSDS

HDAC3 Immunoprecipitation (IP) & Activity Assay Kit

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. These proteins have crucial roles in development and physiology, especially in the heart and nervous system. They are also deeply involved in cellular proliferation, cell cycle and apoptosis. HDAC3 is primarily localized in the nucleus, but can also be found in the cytoplasm and at the plasma membrane. The HDAC3 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC3 complex from cells & tissues and to measure HDAC3 activity fluorometrically. HDA3 is immunoprecipitated from cell or nuclear extract(s) using HDAC3 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.

Figure: (a).AMC Standard Curve (b).HDAC3 IP Activity Assay of Jurkat cell lysate and nuclear extract, mouse brain and liver
extract and HDAC Activity Assay of Positive Control .Assays were performed according to the kit protocol.

Key Information Description

Product SKU

BN00608

Size

25 assays

Detection Method

Fluorescence (Ex/Em=368/442 nm)

Applications

  • Immunoprecipitation of HDAC3 complex from cell & nuclear extract(s)
  • Measurement of HDAC3 activity of immunoprecipitated complex/purified enzyme
  • Screening for activators or inhibitors of HDAC3

Features and Benefits

  • Simple, fast and convenient.
  • Only kit on the market for both IP as well as measuring activity of HDAC3.

Kit Components

  • HDAC Assay Buffer
  • Cell Lysis Buffer
  • HDAC Substrate
  • Developer
  • AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM)
  • Rabbit HDAC2 Antibody (0.2 mg/ml)
  • Rabbit IgG (Control Antibody) (0.5 mg/ml)
  • Protein A/G Sepharose Beads
  • Positive Control (Jurkat Cell Lysate) (125 )

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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