HDAC6 Activity Assay Kit (Fluorometric) (BN00702)

SKU:
BN00702
  • Molecular Biology Tools HDAC6 Activity Assay Kit Fluorometric BN00702
  • Molecular Biology Tools HDAC6 Activity Assay Kit Fluorometric BN00702
  • Molecular Biology Tools HDAC6 Activity Assay Kit Fluorometric BN00702
€787

Description

HDAC6 Activity Assay Kit (Fluorometric)

Histone Deacetylase 6 (HDAC6, EC 3.5.1.98), is a class IIb HDAC enzyme that deacetylates an N-acetyl lysine of histone and non-histone protein substrates. It is a unique member of HDAC family as it contains two deacetylase domains that are proposed to function independent of each other. HDAC6 can shuttle between the nucleus and cytoplasm, suggesting potential extra-nuclear functions by regulating the acetylation status of non-histone substrates. HDAC6 also affects transcription and translation by regulating the heat-shock protein 90 (Hsp90) and stress granules. Elevated HDAC6 activity is associated with cell motility and increases tubulin deacetylation, thus influencing cancer cell metastasis. In addition, mutations in HDAC6 gene have been associated with Alzheimer's disease. HDAC6-selective inhibitors are considered as promising targets for autoimmune, oncology and inflammatory diseases.

Assay Genie's HDAC6 Activity Assay Kit utilizes deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore, which can be easily quantified using a conventional microplate reader. This simple and high-throughput adaptable assay kit can be used to detect HDAC6 activity (as low as 3 U) in complex biological samples.

Figure: AFC Standard Curve (A), HDAC6 activity with different amounts of Positive Control (B), and in rat liver, kidney lysates along with Hela Nuclear Extract, uninduced and Camptothecin-induced Jurkat Cell Extracts (C) are shown in the figure (n = 3). The assays were performed according to the kit protocol.

Key Information Description

Product SKU

BN00702

Size

100 assays

Measurement

Fluorescence (Ex/Em 380/490 nm)

Kit Summary

Rapid, high-throughput screening of drugs and novel ligands. Development of structure-activity relationship (SAR) models to predict HDAC6 interaction liability of novel compounds.

Features and Benefits

  • Simple, highly sensitive, high-throughput compatible
  • Rapid screening of HDAC6 inhibitors
  • Kit includes Tubacin, an HDAC6 inhibitor, and a stable, recombinant human HDAC6

Kit Contents

  • HDAC6 Assay Buffer
  • HDAC6 Lysis Buffer Human
  • HDAC6 Positive Control
  • HDAC6 Substrate Developer
  • HDAC6 Inhibitor (Tubacin, 1 mM)
  • AFC Standard (1 mM)

Storage Conditions

-80°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Molecular Tools
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