Anti-HIST1H3A (Ab-6) Antibody (PACO56711)

Antibody Name:	HIST1H3A (Ab-6) Antibody (PACO56711)
Antibody SKU:	PACO56711
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IP, ChIP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:100-1:1000, IHC:1:20-1:200, IP:1:200-1:2000
Species Reactivity:	Human
Immunogen:	Peptide sequence around site of Thr (6) derived from Human Histone H3.1

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate. All lanes: HIST1H3A antibody at 1.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 kDa. Observed band size: 16 kDa.
	Immunoprecipitating HIST1H3A in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PACO56711 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO56711 (5µg) + Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (20µg).
	IHC image of PACO56711 diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms:

Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ

UniProt Protein Function:	H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:	P68431
NCBI GenInfo Identifier:	55977055
NCBI Gene ID:	8357
NCBI Accession:	P68431.2
UniProt Secondary Accession:	P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1,
UniProt Related Accession:	P68431
Molecular Weight:	15kDa
NCBI Full Name:	Histone H3.1
NCBI Synonym Full Names:	histone cluster 1 H3 family member h
NCBI Official Symbol:	HIST1H3H
NCBI Official Synonym Symbols:	H3/k; H3FK; H3F1K
NCBI Protein Information:	histone H3.1
UniProt Protein Name:	Histone H3.1
UniProt Synonym Protein Names:	Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name:	HIST1H3A

Antibodies	ELISA Kits
Anti-MonoMethyl-Histone H3-K27 Antibody (CAB2361)	Histone H3 Colorimetric Cell-Based ELISA Kit
Anti-Asymmetric DiMethyl-Histone H3-R26 Antibody (CAB2375)	Histone H3 (Phospho-Thr3) Colorimetric Cell-Based ELISA Kit
Anti-Asymmetric DiMethyl-Histone H3-R17 Antibody (CAB2421)
Anti-MonoMethyl-Histone H3-R17 Antibody (CAB3151)
Anti-Symmetric DiMethyl-Histone H3-R17 Antibody (CAB3152)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)