Histamine Assay Kit (Colorimetric) (BN00737)

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SKU:
BN00737
  • Histamine Assay Kit (Colorimetric) (BN00737)
  • Histamine Assay Kit (Colorimetric) (BN00737)
€577

Description

ELISA Kit Technical ManualMSDS

Histamine Assay Kit (Colorimetric)

Histamine is a biogenic amine with considerable biological relevance. As a signaling molecule histamine plays manifold roles in the body, ranging from local immune responses to neurotransmission. Histamine is also frequently encountered in food, particularly fish and fermented food products such as sauerkraut and aged cheeses. This is because some bacteria generate histamine from histidine via the enzyme histidine decarboxylase. Elevated levels of bacterial fermentation can lead to elevated histamine in raw meat and food products. Histamine levels thus can be used as an indicator of spoilage. While the body is accustomed to exposure to low levels (30-50 ppm, or mg/l) of histamine, ingestion of food containing levels higher than several hundred parts per million can lead to adverse effects. This is known as histamine poisoning. Assay Genie's Histamine Assay Kit can thus be used to identify dangerous levels of histamine in various fish and meat products, as well as other fermented food and sauces/beverages. With our kit, histamine can be detected in samples at concentrations as low as 100 , or 10 /ml (10 ppm).

Figure: (a) Histamine Standard Curve. (b) Estimation of Histamine concentration in putrid fish and fish sauce. Fish sample was prepared according to above protocol. Fish sauce was deproteinized using 10 kDa spin colum and diluted 20-fold using Histamine Assay Buffer. Histamine concentrations are: Putrid Fish: 88 ± 8 ppm, Fish Sauce: 847 ± 27 ppm.

Key Information Description

Product SKU

BN00737

Size

100 assays

Detection Method

Colorimetric (OD: 450 nm)

Applications

Estimation of histamine in various biological samples

Features and Benefits

  • Simple, rapid & convenient assay
  • Limit of detection: 10 µM
  • Very specific, does not interact with other bioamines

Kit Components

  • Histamine Assay Buffer
  • Histamine Enzyme Mix
  • HIstamine Probe
  • Histamine Standard (50 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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