Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit (BN00770)

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BN00770
  • Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit (BN00770)
  • Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit (BN00770)
  • Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit (BN00770)
  • Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit (BN00770)
€496

Description

ELISA Kit Technical ManualMSDS

Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit

Peripheral blood mononuclear cells (PBMCs) are found in the peripheral blood circulating throughout the body and not sequestered in the lymphatic system, spleen, liver or bone marrow. PBMCs are composed of four cell types: T cells, B cells, Natural Killer cells and monocytes, each having a round nucleus. Applicable research areas include: stem cell research, evaluation the immunotherapeutic potential of T or B cells and evaluation of inflammatory response orchestrated by cytokines and chemokines. Based on the unique density of each component, the whole blood is separated into plasma, PBMCs, density gradient media and red blood cells (RBCs). Assay Genie's Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit for PBMC Isolation is the first kit available to provide a complete set of components for the isolation of PBMCs from human blood and also allow visualization of viable cells. High yields of PBMCs (.5 x106 cells/ml) have been obtained following this simple and time-saving protocol. By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cell counts (3%).

Figure A. Illustration of conical tube held at appropriate angle while blood layered on top of DGM. Figure B.1. Layers of DGM and whole blood prior to and after centrifugation showing the separation of layers in the conical tube. Figure B.2 illustrates separation of four layers (plasma, PBMCs, DGM and RBCs). Figure C. Brightfield image of hemocytometer showing total cells(left); image from Fluorescent microscope with Rhodamine/FITC filters of same ROI showing live (green) and dead (red) cells (middle); merge of two panels (right).

Key Information Description

Product SKU

BN00770

Size

30 ml isolation

Detection Method

Isolation of PBMCs from human whole blood. Determination of cell viability and also contamination from RBCs in isolated cell fraction.

Species Reactivity

Human

Applications

  • Isolation of PBMCs from human whole blood.
  • Determination of cell viability and also contamination from RBCs in isolated cell fraction.

Features and Benefits

  • PBMC high yields (.5 x10cells/ml)
  • By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cell counts (3%)

Kit Components

  • Density Gradient Media
  • Viability Stain
  • EDTA 0.5, pH 8.0
  • Viability Stain
  • Blunt-end needle 18 G; 1.5 in. (Sterile)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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