Human Phospho-STAT6 (Y641) PharmaGenie ELISA Kit (SBRS1994)

Product type:
  • Human Phospho-STAT6 Y641 PharmaGenie ELISA Kit SBRS1994
  • Human Phospho-STAT6 Y641 PharmaGenie ELISA Kit SBRS1994
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Human Phospho-STAT6 (Y641) PharmaGenie ELISA - Kit Data

Product SKU:SBRS1994
Application:This ELISA kit recognizes Human STAT6 phosphorylated at site Tyrosine-641.
Gene ID:6778
Gene Names:STAT6
Pathway:JAK/STAT Signaling
Synonyms:Signal transducer and activator of transcription 6 (IL-4 Stat)
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.

Human Phospho-STAT6 (Y641) PharmaGenie ELISA Kit (SBES1994) - Product Images

Product ImageA431 cells were treated or untreated with EGF. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageA431 cells were treated with recombinant human EGF at 37°C for 20 min. Cells were solubilzed at 4 x 107 cells/ml in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Anti-Phospho Antibody
4.HRP-Conjugated Secondary Antibody
5.Assay Diluent
6.TMB One-Step Substrate
7.Stop Solution
8.Lysis Buffer
9.Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-STAT6 (Y641) ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1.Prepare all reagents and samples as instructed in the manual.
2.Add 100 ul of sample or positive control to each well.
3.Incubate 2.5 h at RT or O/N at 4 °C.
4.Add 100 ul of prepared primary antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared 1X HRP-Streptavidin to each well.
7.Incubate 1 h at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.