Polymorphonuclear cells (PMN) have a multilobed nucleus and include the following cells types: mast cells, neutrophils, eosinophils and basophils. Upon isolation, these cells can be used for migration assays and determination of cytokine/chemokine production in response to pharmacological compounds. Assay Genie's PMN Cell Isolation Kit is unique in the market because it enables the user to isolate PMNs, assess viability of the obtained cell fractions and also determine purity with three simple, easy-to-use reagents! High yields, or 60-80% of total PMNs have been obtained yielding 5 x106 cells per ml of human whole blood. Purity of the cell fraction is assessed with giemsa stain (included). Assay Genie's kit use results in highly enriched (>95%) cell fraction of polymorphonuclear cells with less than 3% red blood cell counts. More than 99% of the cells are viable, as determined by the Viability Stain

Figures A. Illustration of conical tube held at 45 degrees while blood layered on top of PMN DGM. B.1. Layers of PMN DGM and whole blood prior to and after centrifugation showing the separation of layers in the conical tube. B.2 Illustrates separation of six layers (plasma, PBMCs, PMN DGM, PMNs, PMN DGM and RBCs) obtained after spinning whole blood with PMN DGM. C. Bright-Field image of hemocytometer showing total cells(left); image from Fluorescent microscope with Rhodamine/FITC filters of same ROI showing live (green) and dead (red) cells (middle); merge of two panels (right). D. Polymorphonuclear Cells stained with Giemsa stain indicating purity of cell suspension obtained.