Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric) (BN00922)

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BN00922
  • Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric) (BN00922)
  • Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric) (BN00922)
  • Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric) (BN00922)
€559

Description

ELISA Kit Technical ManualMSDS

Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric)

NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP, EC:1.1.1.80) belongs to the superfamily of alcohol dehydrogenases (Alcohol DHs, ADHs, EC 1.1.1.1) with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Isopropanol dehydrogenase facilitates the conversion between secondary alcohols to aldehydes and ketones with the reduction of NADP+ to NADPH. Assay Genie's Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1-NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.

Figure: (a) NADPH standard curve; (b) ADH1-NADP Positive Controls (µl/assay). Assays were performed following the kit protocol; (c) ~5 mg of bovine liver, yeast and E.coli cell pellet were homogenized as described in the kit protocol. Serial dilutions were tested to ensure the readings were within the linear range of the Standard Curve. ADH1-NADP activities (mU/mg) of samples were measured and calculated as in the kit protocol.

Key Information Description

Product SKU

BN00922

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian samples. Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc.

Applications

Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells.Measurement of NADP-dependent alcohol dehydrogenases' activity with broad substrate specificity in various tissues/cells.

Features and Benefits

  • Simple, colorimetric protocol.
  • Detects ADH1-NADP activity as low as 0.001 mU in samples.
  • Compatible with multiple sample types.

Kit Components

  • ADH1-NADP Assay Buffer
  • ADH1-NADP Substrate
  • ADH1-NADP Developer
  • ADH1-NADP Positive Control
  • NADPH Standard

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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