NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP, EC:1.1.1.80) belongs to the superfamily of alcohol dehydrogenases (Alcohol DHs, ADHs, EC 1.1.1.1) with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Isopropanol dehydrogenase facilitates the conversion between secondary alcohols to aldehydes and ketones with the reduction of NADP+ to NADPH. Assay Genie's Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1-NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.

Figure: (a) NADPH standard curve; (b) ADH1-NADP Positive Controls (µl/assay). Assays were performed following the kit protocol; (c) ~5 mg of bovine liver, yeast and E.coli cell pellet were homogenized as described in the kit protocol. Serial dilutions were tested to ensure the readings were within the linear range of the Standard Curve. ADH1-NADP activities (mU/mg) of samples were measured and calculated as in the kit protocol.