Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001-10 mM of various Lactate samples.

Figure: Lactate Standard Curve: colorimetric (a), fluorometric (b). Quantitation of lactate in human saliva serum. (c)Saliva was centrifuged at 10000 x g for 10 min. at 4°C. 3 µl of supernatant was spiked with a known amount of lactate (4 nmol) as internal Standard. Serum was diluted 20-fold and 3 μl was assayed. Calculated concentrations (mg/dl): Saliva: 0.42 ± 0.05; Serum: 12.9 ± 0.5. Assays were performed according to the kit protocol.