Lactulose Assay Kit (Colorimetric) (BA0128)

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ELISA Kit Technical ManualMSDS

Lactulose Assay Kit

LACTULOSE (C12H22O11) is a synthetic sugar that is non-digestible by the gut. It is used to treat constipation and hyperammonemia. It may also be used for intestinal permeability test for leaky gut. Lactulose can be found in milk-based products. Raw milk normally contains extremely low or no level of lactulose. However, the lactulose level in milk will increase after heat-treated.

Assay Genie's lactulose assay kit is based on beta-galactosidase catalyzed oxidation of lactulose, which generates D-fructose and D-galactose. The generated D-fructose reacts with the specific reagent to form a colored product whose color intensity, measured at 565 nm, is proportional to the lactulose concentration in the sample.


For quantitative determination of lactulose in food, beverage, agricultural products, and biological samples

Key Features

  • Fast and sensitive. Use of 40 uL sample. Linear detection range 3 to 300 uM lactulose in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
  • High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day

Lactulose Assay Kit Information:

Method of Detection: OD565nm
Detection Limit: 3 uM
Samples: Food, beverage, agricultural products and biological samples
Species: All
Protocol Length: 60 min
Size: 100 tests
Shelf Life: 6 months


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