Lactulose Assay Kit (Colorimetric) (BA0128)
Lactulose Assay Kit
LACTULOSE (C12H22O11) is a synthetic sugar that is non-digestible by the gut. It is used to treat constipation and hyperammonemia. It may also be used for intestinal permeability test for leaky gut. Lactulose can be found in milk-based products. Raw milk normally contains extremely low or no level of lactulose. However, the lactulose level in milk will increase after heat-treated.Assay Genie's lactulose assay kit is based on beta-galactosidase catalyzed oxidation of lactulose, which generates D-fructose and D-galactose. The generated D-fructose reacts with the specific reagent to form a colored product whose color intensity, measured at 565 nm, is proportional to the lactulose concentration in the sample.
ApplicationsFor quantitative determination of lactulose in food, beverage, agricultural products, and biological samples
- Fast and sensitive. Use of 40 uL sample. Linear detection range 3 to 300 uM lactulose in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day
Lactulose Assay Kit Information:
|Method of Detection:||OD565nm|
|Detection Limit:||3 uM|
|Samples:||Food, beverage, agricultural products and biological samples|
|Protocol Length:||60 min|
|Shelf Life:||6 months|