LDH Cytotoxicity Fluorometric Assay Kit (BN00581)

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BN00581
  • LDH Cytotoxicity Fluorometric Assay Kit (BN00581)
  • LDH Cytotoxicity Fluorometric Assay Kit (BN00581)
  • LDH Cytotoxicity Fluorometric Assay Kit (BN00581)
€487

Description

ELISA Kit Technical ManualMSDS

LDH Cytotoxicity Fluorometric Assay Kit

Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. Assay Genie's LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.

Figure: (a) Untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min. (high control), (b) Relative fluorescence units of untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min. (high control), (c) Overnight treatment of HeLa cells with 100 µM of staurosporine or 3 µM of cycloheximide. LDH released into the medium was measured along with blank, untreated cells (low control), LDH Positive Control, and lysed cells (high control). Assay was performed according to the kit protocol.

Key Information Description

Product SKU

BN00581

Size

500 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

  • Measurement of released LDH in culture medium
  • Evaluation of cytotoxicity effect of test compounds

Features and Benefits

  • Highly-Sensitive: Detects as less as 100 cells.
  • Rapid protocol: requires less than 20 min.
  • High-throughput adaptable.

Kit Components

  • LDH Assay Buffer
  • LDH Substrate Mix
  • Cell Lysis Solution
  • LDH Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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