Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit (BN00955)

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BN00955
  • Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit (BN00955)
  • Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit (BN00955)
  • Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit (BN00955)
€667

Description

ELISA Kit Technical ManualMSDS

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. Assay Genie's Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (= 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Figure: (a) MDA Standard Curve: Colorimetric (b) and Fluorometric (c) Measurement of MDA in human plasma (20 µl) and rat liver lysate (10 mg). Assay was performed following the kit protocols.

Key Information Description

Product SKU

BN00955

Size

100 assays

Detection Method

Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)

Species Reactivity

All

Applications

The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples

Kit Components

  • MDA Lysis Buffer
  • Phosphotungstic Acid Solution
  • BHT (100X)
  • TBA
  • MDA Standard (4.17 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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