Lipopolysaccharide (LPS) Isolation Kit (BN00681)

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BN00681
  • Lipopolysaccharide (LPS) Isolation Kit (BN00681)
  • Lipopolysaccharide (LPS) Isolation Kit (BN00681)
€371

Description

ELISA Kit Technical ManualMSDS

Lipopolysaccharide (LPS) Isolation Kit

The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous, and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis. Assay Genie's LPS Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chlorofrom or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified!

Figures A. Concentration of LPS in lysates recovered from overnight culture of E.coli were quantified using the Total Carbohydrate Colorimetric Assay Kit. Carbohydrate standards were prepared in LPS Lysis Buffer. B. LPS isolated from E. coli using this protocol was loaded onto a 4-20% gradient SDS gel, run for 55 min at 140 V, and then stained with Coomassie blue protein stain. LPS is a 10-20 kDa carbohydrate that is associated with low molecular weight proteins and gives a characteristic ladder banding pattern in coomassie blue stained SDS-PAGE gels. Lane 1 represents E. coli lysate prior to Proteinase K digestion. Lane 2 illustrates lysate after Proteinase K digestion of proteins. MM: molecular weight marker.

Kit Information Description

Product SKU

BN00681

Size

10 isolations

Kit Summary

Isolation of LPS from outer membrane of gram negative bacteria.

Detection Method

Not Applicable

Species Reactivity

Bacteria

Applications

Isolation of LPS from outer membrane of gram negative bacteria.

Features and Benefits

  • This kit does not use chlorofrom or phenol like traditional methods
  • Yields pure LPS in less than 2 hours that can be easily characterized and quantified

Kit Components

  • LPS Isolation Buffer
  • Proteinase K (20 mg/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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