Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based) (BN00696)

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BN00696
  • Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based) (BN00696)
  • Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based) (BN00696)
€655

Description

ELISA Kit Technical ManualMSDS

Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)

Lysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients, toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease. Assay Genie has developed the Lysosomal Cytotoxicity Dual Staining Kit (cell-based) which contains two probes; membrane permeable, selective Lysosomal Stain and Cell Death Marker. We also include a Positive Control Reagent, which increases lysosome activity and staining, thus serves as an experimental control. This easy-to-use, non-radioactive kit allows studying the regulation of lysosome and cytotoxicity at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells.

Figure: Lysosomal Staining in Jurkat cells. 1x10 6 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hr. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to kit’s protocol. Panel A: Comparison of histograms from FACS analysis showing lysosomal staining. Unstained cells (black); Control cells stained with 1X Lysosomal Staining (green); Cells treated with Positive Control Reagent staining (blue). Panel B: Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

Key Information Description

Product SKU

BN00696

Size

50 assays

Kit Components

  • Detection method- Flow cytometry and Fluorescence Microscopy
  • Species reactivity- Mammalian
  • Applications - Measurement of Lysosomal cytotoxicity- Screening compounds affecting lysosomal function

Detection Method

Flow Cytometry (FL1/FL2)

Species Reactivity

Mammalian

Applications

  • Measurement of lysosomal cytotoxicity. Staining for lysosome cytotoxicity and cell death.
  • Screening for compounds that affect lysosomal function

Features and Benefits

Measurement of lysosomal cytotoxicity.

Kit Components

  • Assay Buffer (10X)
  • Lysosomal Stain (500X)
  • Cell Death Marker (500X)
  • Positive Control Reagent

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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