Lysozyme Activity Assay Kit (Fluorometric) (BN00504)

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SKU:
BN00504
  • Lysozyme Activity Assay Kit (Fluorometric) (BN00504)
  • Lysozyme Activity Assay Kit (Fluorometric) (BN00504)
  • Lysozyme Activity Assay Kit (Fluorometric) (BN00504)
€565

Description

ELISA Kit Technical ManualMSDS

Lysozyme Activity Assay Kit (Fluorometric)

Lysozyme (EC 3.2.1.17), also known as muramidase or N-acetylmuramide glycanhydrolase, is a hydrolase acting on glycosidic bonds. It hydrolyzes the Beta-(1-4)-glucosidic linkage between N-acetyl-muraminic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell wall. Lysozyme is ubiquitously found in a wide range of biological fluids such as tears, saliva and tissues. It exhibits antibacterial, antitumor and immune modulatory activities. Elevated concentrations of lysozyme in urine and serum have been reported in patients suffering leukemia, tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis and nephritis. Assay Genie's lysozyme activity assay kit utilizes the ability of lysozyme to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 360/445 nm). This kit provides a simple, ultra-sensitive assay that can detect as low as 2 µU/ml of Lysozyme activity in a variety of biological samples.

Figure: (a) 4-Methylumbelliferon Standard Curve, results show results from three independent experiments and Measurement of Lysozyme activity in human samples and cell cultured samples. (b) Undiluted pooled serum (10 µl), pooled tears (15 µl), pooled saliva (30 µl) or (c) J774 cells (15 µg), HepG2 cells (80 µg) were incubated with Lysozyme Substrate for 60 min. All assays were performed following kit protocols.

Key Information Description

Product SKU

BN00504

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 360/445 nm)

Applications

Detection of Lysozyme Activity in variety of samples

Features and Benefits

  • Rapid, and convenient
  • The assay can detect Lysozyme activity as low as 2 µU/ml in a variety of biological samples

Kit Components

  • Lysozyme Assay Buffer
  • Lysozyme Stop Buffer
  • Lysozyme Substrate (in DMSO)
  • Lysozyme Positive Control (lyophilized)
  • 4-Methylumbelliferone Standard (5 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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