Malate Colorimetric Assay Kit (BN00861)

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SKU:
BN00861
€565

Description

ELISA Kit Technical ManualMSDS

Malate Colorimetric Assay Kit

L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others. Assay Genie has developed an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (ax = 450 nm). The assay can detect 1 ~ 35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.

Figure 1: L(-) Malate standard curve generated using this kit protocol

Key Information Description

Product SKU

BN00861

Size

100 assays

Detection Methods

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

The assay can detect 1 ~ 35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.

Features and Benefits

  • Simple procedure; takes ~ 1 hour
  • Fast and convenient
  • This kit contains all necessary reagents for a accurate measurement of Malic Acid

Kit Components

  • Malate Assay Buffer
  • Malate Enzyme Mix
  • WST Substrate
  • Malate Standard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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