Malate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00768)

(No reviews yet) Write a Review
SKU:
BN00768
  • Malate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00768)
  • Malate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00768)
  • Malate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00768)
€649

Description

ELISA Kit Technical ManualMSDS

Malate Dehydrogenase Activity Assay Kit (Fluorometric)

Malate Dehydrogenase (MDH) (EC 1.1.1.37) reversibly converts L-Malate into oxaloacetate in the presence of NAD+. In eukaryotic cells, malate dehydrogenase has 2 isoforms, MDH1 and MDH2. MDH1 is cytosolic and participates in the malate-aspartate shuttle, which transports malate into the mitochondria. Malate will then be utilized for the generation of ATP. MDH2, on the other hand, is a mitochondrial enzyme that participates in the citric acid cycle. MDH2 activity is increased in some neurodegenerative diseases such as Alzheimer's disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). Assay Genie's Malate Dehydrogenase Activity Assay Kit (Fluorometric) provides a quick and easy method for monitoring MDH activities in a wide variety of samples. In this assay, MDH converts malate into intermediate products, which will further react with forming a fluorophore (Ex/Em=535/587nm). The kit is simple, sensitive and high-throughput adaptable and can detect as low as 40 µU of MDH activity.

Figure: (a) NADH Standard Curve; (b) Malate Dehydrogenase activities in rat muscle extract (0.011 µg protein), Jurkat cell lysate (0.026 µg protein), Saccharomyces mitochondria (0.05 µg) & MDH positive control; (c) Specific MDH activities in rat muscle extract, Jurkat cell lysate and yeast Mitochondria. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00768

Size

100 assays

Kit Summary

  • Detection method- Fluorescence(535/587 nm)
  • Applications-Measurement of malate dehydrogenase activity in various tissues/cells
  • Analysis of citric acid cycle and malate-asparate shuttle

Detection Method

Fluorescence (535/587 nm)

Species Reactivity

Mammalian

Applications

  • Measurement of malate dehydrogenase activity in various tissues/cells
  • Analysis of citric acid cycle and malate-asparate shuttle

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect Malate Dehydrogenase (MDH) Activity less than 100 µU in various sample types.

Kit Components

  • MDH Assay Buffer
  • MDH Substrate
  • MDH Developer
  • NADH Standard
  • MDH Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose