Maltose and Glucose Colorimetric/Fluorometric Assay Kit (BN00842)

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SKU:
BN00842
€607

Description

ELISA Kit Technical ManualMSDS

Maltose and Glucose Colorimetric/Fluorometric Assay Kit

Glucose (CO FW: 180.16) and Maltose (CHO; FW: 342.3) are the main fuel sources to generate the universal energy molecule ATP. Maltose is the major disaccharide that generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a 1,4-glycosidic linkage, which can be easily converted to two glucoses by D-glucosidase. Glucose oxidase specifically oxidizes free glucose to produce a product that interacts with the glucose probe to generate color and fluorescence. Therefore, glucose or maltose levels in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.) can be easily determined by either colorimetric (spectrophotometry at 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 10 pmol to 10 nmol glucose per assay.

Maltose Standard Curve: Assays were performed using various amounts of Maltose standard according to kit instructions. Open square is maltose with α-D-Glucosidase. Solid square is maltose without α-D-Glucosidase. Triangle is glucose with 2 times nmol of Maltose.

Key Information Description

Product SKU

BN00842

Size

100 assays

Detection Method

Fluorescence Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Application

The assay can detect 10 pmol to 10 nmol glucose per assay.

Features and Benefits

  • Simple procedure; takes ~ 1 hour.
  • Fast and convenient.
  • Kit provides all necessary buffers and reagents for assay of Maltose and Glucose individually in various biological samples.

Kit Components

  • Glucose Assay Buffer
  • Glucose Probe (Lyophilized)
  • Dimethylsulfoxide (DMSO, Anhydrous)
  • D-Glucosidase (Lyophilized)
  • Glucose Enzyme Mix (Lyophilized)
  • Maltose Standard (100 nmol/µl)

Storage Conditions

-20°C

Shipping Conditions

-20°C

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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