Membrane Protein Extraction Kit (BN00537)

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SKU:
BN00537
  • Membrane Protein Extraction Kit (BN00537)
  • Membrane Protein Extraction Kit (BN00537)
€619

Description

ELISA Kit Technical ManualMSDS

Membrane Protein Extraction Kit

The Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins), Assay Genie's kit was designed to not only extract the total cellular membrane proteins, but also purify the plasma membrane proteins specifically. The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

Figure: Membrane protein extraction: ~4x10 7 HeLa cells (A) were lysed in 500 μl homogenization buffer and ~0.5 g rat liver (B) was homogenized in 1 ml homogenization buffer. 10 μl was loaded onto 4-20% gradient gel and stained with Coomassie Blue. 1-Whole cell lysate, 2- Cytosolic portion, 3-Total membrane protein, 4-Plasma membrane protein.

Key Information Description

Product SKU

BN00537

Size

50 assays

Detection Method

Mammalian

Applications

Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • The Membrance Protein Extraction Kit provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells.

Kit Components

  • Homogenize Buffer
  • Upper Phase Solution
  • Lower Phase Solution
  • Protease Inhibitor Cocktail

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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