Methylglyoxal Assay Kit (Colorimetric) (BN00731)

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  • Methylglyoxal Assay Kit (Colorimetric) (BN00731)
  • Methylglyoxal Assay Kit (Colorimetric) (BN00731)


ELISA Kit Technical ManualMSDS

Methylglyoxal Assay Kit

Methylglyoxal (MG, 2-oxopropanal, pyruvic aldehyde) is a highly reactive dicarbonyl compound formed during glycolysis and sugar fermentation. It is an important precursor of advanced glycation end products (AGEs). Endogenous MG is present in many food items and beverages. For example, high levels of MG have been reported in Manuka Honey and some soft drinks. Additionally, cooking and some storage techniques can induce the synthesis of MG, such as coffee brewing, and bread toasting. It has been shown that MG has antimicrobial activity against some bacterial strains, including Streptococcus, H. pylori, E. coli and other bacteria resistant to standard antibiotic treatments. Assay Genie's Methylglyoxal Assay Kit (Colorimetric) enables the detection of MG using a set of engineered enzymes and a chromophore. The reduced chromophore, final product of the assay, produces a stable signal, which can be easily quantified at 450 nm using a microplate reader and its signal is directly proportional to the amount of MG in samples. The assay is simple, specific, reproducible, and can detect as low as 0.5 nmol/well of MG in a 100 µl reaction.

Figure: (a) MG Standard Curve. (b) Measurement of MG in Manuka Honey (MGO 30+) (10 µl; Dilution Factor: 2, in dH 2 O); Manuka Honey (UMF 15+) (10 µl; Dilution Factor: 10, in dH 2 O). All assays were performed following kit protocols.
*According to reference: MG in Manuka Honey (MGO 30+): ≥ 30 mg/kg; MG in Manuka Honey (UMF 15+): ≥ 510 mg/kg.

Key Information Description

Product SKU



100 assays

Kit Summary

  • Detection method- Absorbance (OD 450 nm)
  • Species reactivity- Eukaryotes
  • Applications- This Kit provides a simple in vitro assay for detecting Methylglyoxal levels in biological samples.

Detection Method

Absorbance (OD 450 nm)

Species Reactivity



Measurement of Methylglyoxal in food products

Features and Benefits

  • Simple procedure; takes ~ 2 hours
  • Fast and convenient
  • The assay is sensitive, stable and high-throughput adaptable Useful for the estimation of Methylglyoxal in biological samples

Kit Components

  • MG Assay Buffer
  • Substrate Mix A
  • Substrate Mix B
  • Enzyme Mix A
  • Enzyme Mix B
  • Enzyme Mix C
  • MG Standard (20 mM)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

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