Methyltransferase Activity Assay Kit (Colorimetric) (BN01144)

(No reviews yet) Write a Review
SKU:
BN01144
€738

Description

ELISA Kit Technical ManualMSDS

Methyltransferase Activity Assay Kit (Colorimetric)

Methyltransferases are a diverse group of enzymes that methylate proteins, DNA, and small molecule targets. Accordingly, methyl transferase activity plays a critical role in modulating gene transcription, protein interactions, and signaling networks. Anomalous DNA methylation patterns have been consistently detected during cancer development as well as in many genetic disorders such as Fragile X Syndrome, ICF, and Rett Syndrome. Assay Genie's Methyltransferase Activity Assay Kit (Colorimetric) allows for kinetic evaluation of methyltransferase activity of purified enzymes and their inhibitors. The transfer of a methyl group from S-Adenosyl Methionine (SAM) cofactor to a corresponding substrate generates S-Adenosyl Homocysteine (SAH) as a product. SAH is detected by coupling the methyl transfer reaction to a multi-step enzymatic cascade, resulting in the generation of an intermediate that reacts with GenieRed™ Probe. The reaction product exhibits a strong absorbance at 570 nm. The limit of quantification (L.O.Q) is 296 pmol of SAH generated per min per ml (296 µU/ml) of purified enzymes.

Figure: a) Normalized SAH Standard Curve; b) Representative activity curve for human recombinant Nicotinamide N-Methyltransferase (NNMT) with Nicotinamide Substrate at 37°C. Assays were performed using kit protocol.

Key Info Description

Product SKU

BN01144

Size

100 assays

Detection Method

Colorimetric (OD 570 nm)

Species Reactivity

Mammalian

Applications

Fast and convenient

Features & Benefits

  • Simple, rapid & convenient assay to measure Methyltransferase Activity in purified enzyme
  • Includes Positive Control

Kit Components

  • MT Assay Buffer
  • Enzyme Re-suspension Buffer
  • Enzyme Mix I
  • Enzyme Mix II
  • Enzyme Mix III
  • SAM Cofactor (50 mM)
  • SAH Standard (50 mM)
  • MT Positive Control
  • GenieRed Probe

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans.

View AllClose