Description
Methyltransferase Activity Assay Kit (Colorimetric)
Methyltransferases are a diverse group of enzymes that methylate proteins, DNA, and small molecule targets. Accordingly, methyl transferase activity plays a critical role in modulating gene transcription, protein interactions, and signaling networks. Anomalous DNA methylation patterns have been consistently detected during cancer development as well as in many genetic disorders such as Fragile X Syndrome, ICF, and Rett Syndrome. Assay Genie's Methyltransferase Activity Assay Kit (Colorimetric) allows for kinetic evaluation of methyltransferase activity of purified enzymes and their inhibitors. The transfer of a methyl group from S-Adenosyl Methionine (SAM) cofactor to a corresponding substrate generates S-Adenosyl Homocysteine (SAH) as a product. SAH is detected by coupling the methyl transfer reaction to a multi-step enzymatic cascade, resulting in the generation of an intermediate that reacts with GenieRed Probe. The reaction product exhibits a strong absorbance at 570 nm. The limit of quantification (L.O.Q) is 296 pmol of SAH generated per min per ml (296 µU/ml) of purified enzymes.
Figure: a) Normalized SAH Standard Curve; b) Representative activity curve for human recombinant Nicotinamide N-Methyltransferase (NNMT) with Nicotinamide Substrate at 37°C. Assays were performed using kit protocol.
Key Info | Description |
Product SKU | BN01144 |
Size | 100 assays |
Detection Method | Colorimetric (OD 570 nm) |
Species Reactivity | Mammalian |
Applications | Fast and convenient |
Features & Benefits |
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Kit Components |
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Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
Usage | For Research Use Only! Not For Use in Humans. |