Mitochondrial Apoptosis Detection Fluorometric Kit (BN00515)

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SKU:
BN00515
€565

Description

ELISA Kit Technical ManualMSDS

Mitochondrial Apoptosis Detection Fluorometric Kit

Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoCapture accumulates and aggregates in the mitochondria, giving off a bright red fluorescence. In apoptotic cells, MitoCapture cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530 + 30 nm) and (optional) PI channel for red aggregates (Ex/Em = 488/590 + 42 nm).

Key Information Description

Detection Method

Fluorescence microscopy and Flow cytometry: FITC (Ex/Em = 488/530 ± 30 nm); and (optional) PI (Em = 488/590 ± 42 nm)

Size Options

  • 25 Assays
  • 100 Assays

Species Reactivity

Mammalian

Applications

Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. It is highly sensitive and detects apoptosis in living cells.

Features and Benefits

  • Simple one-step procedure; takes only 30 minutes
  • Fast and convenient

Kit Components

  • MitoCapture Reagent
  • Incubation Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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Additional Information

Product type:
Assay
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