Mitochondrial DNA Purification Kit (BN00647)

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SKU:
BN00647
€517

Description

ELISA Kit Technical ManualMSDS

Mitochondrial DNA Purification Kit

Mitochondria are essential double-membrane bound organelles that support aerobic respiration and most of the cellular ATP production in all nucleated mammalian cells. To date, hundreds of pathogenic mitochondrial DNA mutations have been identified to result in disease phenotypes such as cancer, ataxia, and diabetes. As a result, isolation of high quality mitochondrial DNA from mammalian cells and tissues in sufficient quantities is important for many basic research and clinical applications. Assay Genie's Mitochondrial DNA Isolation Kit facilitates purification of quality mitochondrial DNA from mammalian cultured cells or tissue. This kit utilizes a density separation step to isolate mitochondria from the rest of the cellular organelles, followed by an enzymatic reaction to release DNA from the mitochondria. Upon release, mitochondrial DNA is further purified from nuclear DNA contamination and adsorbed onto a silica spin-column under chaotropic conditions, eliminating the use of toxic organic compounds or solvents. Mitochondrial DNA purified by this kit is suitable for various downstream molecular biology applications such as PCR, cloning, DNA hybridization, Southern Blotting, sequencing, genotyping, enzymatic analysis, and more.

Figure 1: Mitochondrial DNA detection by electrophoresis: Lane 1 – 1 kb DNA Ladder; Lane 2 – undigested human mtDNA
purified by this kit; Lane 3 – human mtDNA digested with BamHI; Lane 4 – rat mtDNA amplified by PCR using rat-specific mtDNA primers; Lane 5 – human mtDNA amplified by PCR using human-specific mtDNA primers.

Key Information Description

Product SKU

BN00647

Size

25 purifications

Detection Method

Species reactivity- Mammalian PCR, cloning, DNA hybridization, southern blotting, genotyping, restriction enzyme digest analysis, sequencing, etc.

Species Reactivity

Mammalian

Applications

PCR, cloning, DNA hybridization, southern blotting, genotyping, restriction enzyme digest analysis, sequencing, etc.

Features and Benefits

  • Simple procedure; takes less than 2 hours
  • Fast and convenient
  • The kit provides unique formulations of buffers, reagents anc columns for isolating a highly enriched mitochondrial fraction.
  • This kit provides genomic-free DNA samples

Kit Components

  • Buffer H [Homogenization Buffer]
  • Buffer R [Re-suspension Buffer]
  • Enzyme Mix
  • RNAse A
  • Buffer L [Lysis Buffer]
  • Buffer N [Neutralization Buffer
  • Buffer W [Wash Buffer]
  • Buffer E [Elution Buffer]
  • Spin Columns/Collection Tubes

Storage Conditions

-20°C/RT

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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