MMP-3 Inhibitor Screening Kit (Fluorometric) (BN01009)

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BN01009
  • MMP-3 Inhibitor Screening Kit (Fluorometric) (BN01009)
  • MMP-3 Inhibitor Screening Kit (Fluorometric) (BN01009)
$775.60

Description

ELISA Kit Technical ManualMSDS

MMP-3 Inhibitor Screening Kit (Fluorometric)

The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In Assay Genie's MMP-3 Inhibitor Screening Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Em/Ex=325/393 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of MMP-3 inhibition. In addition, we also offer a human recombinant MMP-3 enzyme (Assay Genie #7783) and a MMP-3 Activity Assay Kit (Assay Genie #K783-100), separately.

              (a)

(b)

Figure: (a) MMP-3 activity vs [GM6001] (b) Relative Activity graph

Key Information Description

Product SKU

BN01009

Size

100 assays

Detection Method

Fluorescence (Ex/Em 325/393 nm)

Applications

The kit can be used to screen inhibitors of MMP-3.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • MMP-3 Assay Buffer
  • MMP-3 Substrate
  • MMP-3 Enzyme (lyophilized)
  • Inhibitor Control (0.1 mM GM6001)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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