Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (BN01012)

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SKU:
BN01012
  • Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (BN01012)
  • Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (BN01012)
€732

Description

ELISA Kit Technical ManualMSDS

Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity, and tissue localization. MAO-A can oxidize primary amines such as serotonin and norepinephrine. MAO-A is a mitochondrial-bound enzyme that is ubiquitously expressed throughout the brain and other tissues. It has been implicated in panic, anxiety, and depression. Several MAO-A specific inhibitors such as clorgyline, brofaromine, toloxatone, tetrindole, etc. have been used as antidepressants, but their usage has been limited due to side effects. Assay Genie's MAO-A Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate.

Figure: (a) Inhibition of MAO-A Activity with Clorgyline. (b) Clorgyline does not inhibit MAO-B activity (an isozyme of MAO-A). Assays were performed following kit protocol.

Key Information Description

Product SKU

BN01012

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

Screening/studying/characterizing MAO-A inhibitors

Features and Benefits

  • Rapid & sensitive
  • High-throughput assay to screen/study/characterize potential inhibitors of monoamine oxidase A
  • Includes Inhibitor Control [Clorgyline]

Kit Components

  • MAO-A Assay Buffer
  • GenieRed Probe (in DMSO)
  • MAO-A Enzyme (Lyophilized)
  • MAO-A Substrate (Lyophilized)
  • Developer (Lyophilized)
  • Inhibitor Control (Clorgyline) (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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