Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit (BN01011)

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BN01011
  • Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit (BN01011)
  • Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit (BN01011)
  • Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit (BN01011)
€553

Description

ELISA Kit Technical ManualMSDS

Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity and tissue localization. Clonogenic studies have shown that these two isozymes have similar catalytic characteristics, yet their amino acid sequences are different. MAO-A favors Serotonin, Norerpinephrine and Dopamine as substrates, while phenylethylamine and benzylamine are MAO-B preferred substrates. MAO-A and MAO-B are mitochondrial-bound enzymes that are ubiquitously expressed throughout the brain and other tissues. Imbalance of MAOs levels has been associated with schizophrenia, depression, attention deficit and other disorders. MAO-A has been implicated in panic, anxiety and depression, whereas MAO-B defects result in Alzheimer's and Parkinson's diseases. Assay Genie's Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) assay is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAO-B, respectively. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of the MAO substrate. The assay can detect as little as 5 µU of MAO enzymatic activity.

      (a)

        (b)

                (c)

Figure: (a) H2O2 Standard Curve. (b) MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 μg) (c) Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.

Key Information Description

Product SKU

BN01011

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

Measurement of Total MAO, MAO-A and MAO-B activities in various biological samples.

Features and Benefits

  • Rapid & Convenient
  • The assay can detect as little as 5 µU of MAO enzymatic activity.
  • Includes MAO-A specific Inhibitor Clorgyline, MAO-B specific
  • Inhibitor Selegiline & MAO-A Enzyme as Positive Control

Kit Components

  • MAO Assay Buffer
  • GenieRed Probe (in DMSO)
  • MAO Substrate (Lyophilized)
  • Developer (Lyophilized)
  • Positive Control (MAO-A Enzyme) (Lyophilized) (Lyophilized)
  • MAO-A Inhibitor, Clorgyline (Lyophilized)
  • MAO-B Inhibitor, Selegiline (Lyophilized)
  • H2O2 Standard (0.88 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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