Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (BN01013)

  • Cell Biology Assays Monoamine Oxidase B MAO-B Inhibitor Screening Kit Fluorometric BN01013
  • Cell Biology Assays Monoamine Oxidase B MAO-B Inhibitor Screening Kit Fluorometric BN01013


ELISA Kit Technical ManualMSDS

Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric)

Monoamine oxidases (MAO, EC are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity, and tissue localization. MAO-B can oxidize primary amines, but its list of specific substrates (i.e. benzylamine, phenylethylamine) is more limited compared to MAO-A. MAO-B is a mitochondrial-bound enzyme that is ubiquitously expressed throughout the brain and other tissues. It has been investigated in numerous studies including Parkinson's disease, Alzheimer's, and tobacco addiction. Specific MAO-B inhibitors such as selegiline, & rasagiline have been used to treat Parkinson's patients, but their benefits are considered rather modest. Assay Genie's MAO-B Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate.



Figure: (a) Inhibition of MAO-B Activity with Selegiline. (b) Selegiline does not inhibit MAO-A activity (an isozyme of MAO-B). Assays were performed following the kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)


Screening/studying/characterizing MAO-B inhibitors

Features and Benefits

  • Rapid & sensitive
  • High-throughput assay to screen/study/characterize potential inhibitors of monoamine oxidase B
  • Includes Inhibitor Control [Selegiline]

Kit Components

  • MAO-B Assay Buffer
  • GenieRed Probe (in DMSO)
  • MAO-B Enzyme (Lyophilized)
  • MAO-B Substrate (Lyophilized)
  • Developer (Lyophilized)
  • Inhibitor Control (Clorgyline) (Lyophilized)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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