Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (BN01013)

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BN01013
  • Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (BN01013)
  • Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (BN01013)
€732

Description

ELISA Kit Technical ManualMSDS

Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric)

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity, and tissue localization. MAO-B can oxidize primary amines, but its list of specific substrates (i.e. benzylamine, phenylethylamine) is more limited compared to MAO-A. MAO-B is a mitochondrial-bound enzyme that is ubiquitously expressed throughout the brain and other tissues. It has been investigated in numerous studies including Parkinson's disease, Alzheimer's, and tobacco addiction. Specific MAO-B inhibitors such as selegiline, & rasagiline have been used to treat Parkinson's patients, but their benefits are considered rather modest. Assay Genie's MAO-B Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate.

              (a)

(b)

Figure: (a) Inhibition of MAO-B Activity with Selegiline. (b) Selegiline does not inhibit MAO-A activity (an isozyme of MAO-B). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN01013

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

Screening/studying/characterizing MAO-B inhibitors

Features and Benefits

  • Rapid & sensitive
  • High-throughput assay to screen/study/characterize potential inhibitors of monoamine oxidase B
  • Includes Inhibitor Control [Selegiline]

Kit Components

  • MAO-B Assay Buffer
  • GenieRed Probe (in DMSO)
  • MAO-B Enzyme (Lyophilized)
  • MAO-B Substrate (Lyophilized)
  • Developer (Lyophilized)
  • Inhibitor Control (Clorgyline) (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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