Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (BN00960)

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BN00960
  • Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (BN00960)
  • Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (BN00960)
  • Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (BN00960)
€631

Description

ELISA Kit Technical ManualMSDS

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In Assay Genie's MPO Assay Kit, MPO catalyzes the production of NaClO from Hand NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 485/525 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 per well.

(a)

(b)

(c)

Figure: a) Fluorescein Standard Curve. b) Measurement of MPO activity in WBC lysate (0.1 µg), and MPO Positive Control (3 µl). c) MPO specific activity in WBC lysate. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00960

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 485/525 nm)

Species Reactivity

Mammalian

Applications

N/A

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assays for measuring MPO as low as 0.5 per well

Kit Components

  • Assay Buffer
  • MPO Substrate Stock
  • MPO Probe
  • Fluorescein Standard (1 mM)
  • MPO Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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