NAD/NADH Quantitation Colorimetric Kit (BN00603)

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SKU:
BN00603
  • NAD/NADH Quantitation Colorimetric Kit (BN00603)
  • NAD/NADH Quantitation Colorimetric Kit (BN00603)
  • NAD/NADH Quantitation Colorimetric Kit (BN00603)
€720

Description

ELISA Kit Technical ManualMSDS

NAD/NADH Quantitation Colorimetric Kit

Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. Assay Genie's NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.

Figure: (a) Standard Curve. (b) Quantitation of NADH in rat liver (1.5 mg) and kidney (1.5 mg) homogenate. Tissues were washed with cold PBS followed by homogenization using NADH/NAD Extraction Buffer. Homogenates were centrifuged and deproteinized using a 10 kDa spin column (10000 x g, 10 min, 4°C). Filtrates were spiked with NADH (60 pmol) and assayed according to the protocol. (c) Quantitation of NADH in human serum (30 µl) and urine (30 µl).

Key Information Description

Product SKU

BN00603

Size

100 assays

Detection Methods

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

  • Assay Genie's NADH/NAD
  • Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.

Features and Benefits

  • Simple procedure; takes ~2 hours
  • Fast and convenient
  • Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.

Kit Components

  • NADH/NAD Extraction Buffer
  • NAD Cycling Buffer
  • NAD Cycling Enzyme Mix
  • NADH Developer
  • Stop Solution
  • NADH Standard (MW:763)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use In Humans.

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