The Otog Polyclonal Antibody (PAC060773) is a valuable tool for researchers studying the Otog protein, a key component in the auditory system. This polyclonal antibody, produced in rabbits, is highly specific to human samples and is well-suited for use in Western blot applications. By targeting the Otog protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an excellent choice for investigations in auditory biology and hearing-related disorders.
Otog is known for its involvement in the development and function of hair cells in the inner ear, playing a crucial role in auditory signal transduction. Research focusing on Otog can provide insights into the mechanisms underlying hearing loss, balance disorders, and other auditory impairments. By studying the function of Otog, researchers can potentially uncover new targets for therapeutic interventions aimed at treating these conditions and improving overall auditory function.
IHC image of PACO60773 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO60773 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Glycoprotein specific to acellular membranes of the inner ear. May be required for the anchoring of the otoconial membranes and cupulae to the underlying neuroepithelia in the vestibule. May be involved in the organization and/or stabilization of the fibrillar network that compose the tectorial membrane in the cochlea. May play a role in mechanotransduction processes (By similarity).
Synonyms:
Otogelin, OTOG, OTGN
UniProt Protein Function:
Glycoprotein specific to acellular membranes of the inner ear. May be required for the anchoring of the otoconial membranes and cupulae to the underlying neuroepithelia in the vestibule. May be involved in the organization and/or stabilization of the fibrillar network that compose the tectorial membrane in the cochlea. May play a role in mechanotransduction processes ().
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a component of the acellular membranes of the inner ear. Disruption of the orthologous mouse gene shows that it plays a role in auditory and vestibular functions. It is involved in fibrillar network organization, the anchoring of otoconial membranes and cupulae to the neuroepithelia, and likely in sound stimulation resistance. Mutations in this gene cause autosomal recessive nonsyndromic deafness, type 18B. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
