PAF Acetylhydrolase Activity Assay Kit (Colorimetric) (BN00978)

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SKU:
BN00978
  • PAF Acetylhydrolase Activity Assay Kit (Colorimetric) (BN00978)
  • PAF Acetylhydrolase Activity Assay Kit (Colorimetric) (BN00978)
  • PAF Acetylhydrolase Activity Assay Kit (Colorimetric) (BN00978)
€517

Description

ELISA Kit Technical ManualMSDS

PAF Acetylhydrolase Activity Assay Kit (Colorimetric)

Platelet-Activating Factor (PAF or 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is an important phospholipid mediator, which has diverse biological activities. PAF is synthesized and secreted by a variety of cells such as mast cells, monocytes, macrophages etc. Upregulated PAF signaling can cause pathological inflammation and also has been found to be responsible for sepsis, shock, and traumatic injury. PAF Acetylhydrolase (PAF-AH or 1-alkyl-2-acetylglycerophosphocholine esterase or Lipoprotein-associated phospholipase A2 or Lp-PLA2) (EC 3.1.1.47) hydrolyzes PAF by removing acetyl group at the sn-2 position and converts PAF into biologically inactive form, lyso-PAF. PAF-AH has two forms: extracellular and intracellular that shares some similarities. In human, PAF-AH deficiency leads to severe asthma. Therefore early detection of PAF-AH activity is critical for mechanistic study, diagnosis, prevention, and therapeutic purpose. Assay Genie's PAF Acetylhydrolase Assay kit provides a quick and easy way for monitoring PAF-AH activity in a variety of samples. In this kit, PAF-AH converts PAF into an inactivated form and probe gets converted into a colored product with strong absorbance at 412 nm. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1mU.

Figure: (a) TNB Standard Curve. (b) Measurement of PAF-AH activity in human serum. (c) Relative PAF-AH Activity was calculated in human serum (20 µl) and lysates prepared from rat kidney (75 µg) and liver (45 µg). Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN00978

Size

100 Assays

Detection Method

Absorbance (OD 412 nm)

Applications

  • Measurement of PAF-AH activity in various samples
  • Study/characterize PAF-AH inhibitors or activatorsMechanistic study of inflammatory disorders

Features and Benefits

  • Rapid, simple & convenient
  • This assay kit can detect PAF Acetylhydrolase activity as low as 0.1 mU in a variety of samples

Kit Components

  • PAF-AH Assay Buffer
  • PAF-AH Substrate
  • DTNB Probe (in DMSO)
  • TCEP
  • PAF-AH Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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