pCAF Inhibitor Screening Kit (Fluorometric) (BN00609)

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SKU:
BN00609
€655

Description

ELISA Kit Technical ManualMSDS

pCAF Inhibitor Screening Kit (Fluorometric)

pCAF (P300/CBP-associated factor, EC 2.3.1.48) also known as K (lysine) acetyltransferase 2B (KAT2B), possesses intrinsic histone acetyltransferase (HAT) activity. pCAF contributes to transcriptional activation by acetylating chromatin and transcription factors through its HAT activity. Deregulated HAT activity plays an important role in various cancers. pCAF inhibitors have potential applications in cancer therapy. pCAF uses both histone 3 (H3) and histone 4 (H4) as substrates. Assay Genie's pCAF Inhibitor Screening Kit utilizes H3 peptide and Acetyl CoA as the substrates. pCAF acetylates the peptide and generates Coenzyme A with a free thiol group. The CoA is detected using a Thiol Detecting Probe that reacts with thiol groups and gives enhanced fluorescence that can be measured at Ex/Em = 392/482 nm. In the presence of pCAF specific inhibitors, the enzymatic activity is inhibited resulting in decreased or total loss of fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential pCAF inhibitors.

Figure 1: Inhibition of pCAF activity by the pCAF Inhibitor [Garcinol] (Cat. # 2088). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00609

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 392/482 nm)

Applications

Screening/studying/characterizing potential inhibitors of pCAF

Features and Benefits

  • Simple procedure; takes ~ 45 min
  • Fast and convenient
  • Kit contains the necessary reagents for testing putative pCAF inhibitors

Kit Components

  • pCAF Assay Buffer
  • pCAF Enzyme
  • Acetyl CoA (Lyophilized)
  • H3 Peptide (Lyophilized)
  • Thiol Detecting Probe
  • pCAF Inhibitor (Garcinol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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