PEP Colorimetric/Fluorometric Assay Kit (BN00628)

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  • PEP Colorimetric/Fluorometric Assay Kit (BN00628)
  • PEP Colorimetric/Fluorometric Assay Kit (BN00628)


ELISA Kit Technical ManualMSDS

PEP Colorimetric/Fluorometric Assay Kit

Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase. Assay Genie's PEP Assay Kit provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (ax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.

Figure: Standard Colorimetric/Fluorometric curves obrained using kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity



The detection limit is approximately 1 µM PEP in biological samples.

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • The assay is sensitive, stable and high-throughput adaptable.

Kit Components

  • PEP Assay Buffer
  • PEP Probe (in DMSO)
  • PEP Converter
  • PEP Developer Mix
  • PEP Standard (1 ole)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

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